Corneal Organ Culture Model for Assessing Epithelial Responses to Surfactants
نویسندگان
چکیده
منابع مشابه
Organ culture preservation for corneal tissue. Technical and quality aspects.
INTRODUCTION The technical and quality aspects of organ culture as a storage method for human donor corneas are described. MATERIALS AND METHODS Data electronically stored since 1989 of > 41,000 corneas, processed in the Cornea Bank Amsterdam, are analysed. The technical information of eye banks collected in the Directory of the European Eye Bank Association (EEBA) is used as comparison. Euro...
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Purpose: Tenascin is an extracellular matrix glycoprotein which is expressed transiently in wound healing in human skin and animal corneas. In virro and ednbryological studies suggest that tenascin mediates cell adhesion/anti-adhesion, enhancunen~mlubltion of cell proliiation, and promotion of cell migration. At present there are no published data regarding human comeal wound healing, therefore...
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BACKGROUND/AIM The consequences of fungal contamination of an organ cultured cornea, though exceptional, are often disastrous for the recipient. Consequently, eye banks often quarantine corneas for 10 days or more before passing them for grafting. This period, though detrimental to the endothelial cell density of the delivered cornea, is necessary to detect contamination using conventional micr...
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PURPOSE To develop a new method to investigate proteolysis in the intact lens in organ culture. METHODS Intact mouse lenses were assayed at regular intervals for proteolytic activity using fluorogenic peptide substrates +/- addition of ionomycin. Specific inhibitors were used to determine the activity of calpains, the proteasome and acid lysosomal enzymes. RESULTS Significant levels of prot...
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ژورنال
عنوان ژورنال: Toxicological Sciences
سال: 2000
ISSN: 1096-0929
DOI: 10.1093/toxsci/58.2.306